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Trypsin EDTA solution is a widely used reagent in cell culture applications, primarily for the detachment of adherent cells from culture vessels. It is a combination of trypsin, an enzyme that digests proteins, and EDTA, a chelating agent that binds divalent cations, which are essential for cell adhesion. This solution helps to maintain cell viability while facilitating the passage and subculturing of cells in laboratory settings.
| Composition | Trypsin and EDTA in a buffered saline solution |
| Concentration | Typically 0.25% to 1% trypsin with 0.02% to 0.5% EDTA |
| pH range | Typically between 7.2 and 7.6 |
| Storage conditions | Store at -20°C or -80°C for long-term storage |
| Shelf life | Generally 12 to 24 months when stored properly |
When selecting a Trypsin EDTA solution, consider the concentration of trypsin and EDTA that best suits your cell type and application. It is also important to specify the pH and storage conditions to ensure product stability. Buyers should inquire about certifications, such as sterility and endotoxin levels, and provide estimated quantities needed for their specific applications.
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It is used to detach adherent cells from culture surfaces.
It should be stored at -20°C or -80°C for long-term use.
It is suitable for many adherent cell types but may not be optimal for all.
Typical concentrations range from 0.25% to 1%.
Once opened, it is best to use it within a few weeks to months, depending on storage conditions.
Most commercial solutions are sterilized, but always check the product specifications.