Product type
Dulbecco's Modified Eagle Medium (DMEM) is a widely used cell culture medium formulated to support the growth of various mammalian cells in vitro. It is composed of a balanced mixture of amino acids, vitamins, inorganic salts, glucose, and phenol red, which provides essential nutrients and a stable environment for cell proliferation. DMEM can be supplemented with additional components such as serum or growth factors to enhance its effectiveness for specific cell types. DMEM is typically used in research laboratories, biotechnology, and pharmaceutical industries for applications such as tissue culture, vaccine production, and cell line maintenance. Its versatility and adaptability make it a standard choice for many cell culture protocols.
| Formulation | Amino acids, vitamins, salts, glucose |
| pH range | 6.8 to 7.4 |
| Osmolality | 280 to 320 mOsm/kg |
| Supplementation | Can be supplemented with serum or growth factors |
| Storage conditions | 2 to 8°C |
| Shelf life | Typically 6 to 12 months when stored properly |
When selecting Dulbecco's Modified Eagle Medium (DMEM), consider the specific cell types you will be culturing and whether any additional supplements are necessary for optimal growth. It's important to specify the required formulation, pH, and any specific certifications or quality standards needed for your application. Additionally, provide information on the quantities needed, as bulk purchasing may be available for larger laboratories or production facilities.
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DMEM is suitable for a wide range of mammalian cell types, including fibroblasts, epithelial cells, and some suspension cells.
Yes, DMEM can be adapted for serum-free culture by using appropriate supplements.
DMEM should be stored at 2 to 8°C and protected from light.
The typical pH range for DMEM is between 6.8 and 7.4.
Yes, DMEM can be used for long-term cultures, but it may require regular media changes and supplementation.
Common supplements include fetal bovine serum (FBS), L-glutamine, and antibiotics.