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Dulbecco's Modified Eagle Medium (DMEM) is a widely used cell culture medium formulated to support the growth of various mammalian cells in vitro. It is a modification of the original Eagle's Medium, enriched with essential nutrients, amino acids, vitamins, and glucose, which are vital for cell proliferation and maintenance. DMEM is typically used in laboratories for research and development in the fields of biotechnology, pharmaceuticals, and regenerative medicine.
| Composition | Amino acids, vitamins, glucose, salts |
| pH range | 7.0 to 7.4 |
| Osmolality | 280 to 320 mOsm/kg |
| Storage temperature | 2 to 8┬░C |
| Form | Liquid |
When selecting DMEM, buyers should consider the specific cell lines they intend to culture, as different cell types may require tailored formulations. It is important to specify any additional supplements or serum that may be needed, as well as the desired volume and packaging options. Additionally, buyers should inquire about the medium's expiration date and storage conditions to ensure optimal performance.
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DMEM is used for culturing a variety of mammalian cells in laboratory settings.
Yes, DMEM can be supplemented with serum, hormones, or other growth factors depending on the cell line.
DMEM should be stored at 2 to 8┬░C to maintain its stability and efficacy.
The pH of DMEM typically ranges from 7.0 to 7.4.
DMEM is suitable for many cell types, but specific formulations may be required for certain cells.
The shelf life of DMEM varies by manufacturer, but it is generally best used within a few months of purchase.