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Fetal Bovine Serum (FBS) is a common supplement used in cell culture media to provide essential growth factors, hormones, and nutrients for the cultivation of mammalian cells. It is derived from the blood of fetal calves and is known for its rich composition, which supports cell growth and proliferation. FBS is widely utilized in various research and industrial applications, particularly in the fields of biotechnology and pharmaceuticals.
| Source | Derived from fetal bovine blood |
| Typical volume | 500 mL to 1 L |
| Storage temperature | Stored at -20┬░C or colder |
| pH range | 6.5 to 7.5 |
| Endotoxin level | Typically < 10 EU/mL |
| Mycoplasma testing | Tested negative for mycoplasma contamination |
When selecting Fetal Bovine Serum, buyers should consider the source and quality of the serum, as well as any specific certifications or testing results, such as endotoxin levels and mycoplasma testing. It's important to specify the desired volume and any particular requirements for the serum, such as heat-inactivation or specific country of origin. Quantities can vary based on the scale of the project, so understanding the application will help in determining the amount needed.
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FBS is known for its superior growth-promoting properties compared to other sera, making it a preferred choice for many applications.
FBS should be stored at -20┬░C or colder to maintain its quality and effectiveness.
When stored properly, FBS typically has a shelf life of 5 years.
Yes, FBS can be heat-inactivated to reduce the risk of complement activity, which may interfere with certain cell culture applications.
FBS is suitable for a wide range of mammalian cell types, but specific cell lines may have unique requirements.
Buyers should consider sourcing from suppliers that adhere to ethical practices in the collection of fetal bovine blood.